Their capacity up to 164 samples per 24 hrs for 3 or 656 samples per 24 hrs for 3xl, respectively and at the same time possibility to analyze as few as 4 or 16 samples in a single run make them ideal for low to midthroughput labs. Improper boot sequence can lead to communication issues, aborted runs, and possible loss of d. Software the applied biosystems 3 and 3 xl genetic analyzers include software for data collection and autoanalysis of sample files. Biosystems 33xl genetic analyzer data collection software. On going validation of the 3500xl genetic analyzer using the. For comparison of energy usage when performing a run, the 3500xl instrument was set up to run on nominal voltage, 117. The 3500 series data collection software integrates seamlessly with several downstream software packages to provide comprehensive analysis of genetic data. As part of instrument maintenance, you must change or replenish. Applied biosystems 33xl genetic analyzers maintenance and troubleshooting guide. Hitachi abi prism 3xl genetic analyzer 16 capillary. Applied biosystems hitachi 3xl genetic analyzer dna. The applied biosystems 33xl genetic analyzer system includes both the instrument and the computeryou cannot start or restart one without starting the other. Comes with computer with applicable software and all hardware and cabling. Igt 5dye matrix standard instruction manual version 3.
Below are some of the key features and benefits of the abi 3xl. Perform comparative sequencing, linkage analysis, str analysis, snp detection, discovery and validation, mutation detection, and many other applications. The applied biosystems 3 3 xl genetic analyzer system includes both the instrument and the computeryou cannot start or restart one without starting the other. Designed for mediumthroughput applications, it is capable of sequencing and fragment analysis including microsatellite, aflp, loh, and snp validation and screening. Hitachi abi prism 3xl genetic analyzer 16 capillary unit with computerkey features 16 capillaries for the 3xl and 4 capillaries for the 3 system 24hour unattended operation easy instrument setup polymer filling via the automated polymer deliver system detection cell heater for improved thermal control automated sample injection from both 96 and 384well. See users manual or package insert for limited label license, and trademark information. Fully wet tested see picturespaperwork and instrument shown are for example only and certified by our oem engineers. This versatile platform delivers performance with higher data quality, improved automation and easeofuse, faster turnaround times and higher reliability across the complete range of sequencing, resequencing, and fragment analysis applications. It is taking a longer time than normal to start the applied biosystems 3 3xl genetic analyzer data collection software, what should i do. It can process samples from 96 or 384 well plates, with mixed applications run on a single array. A photo of a capillary array as it appears inside the abi 3xl instrument is shown in figure 6. New kb basecaller software tools provide quality scores for sample sequence. Hitachi abi prism 3xl genetic analyzer 16 capillary unit. The 3500xl genetic analyzer has increased the number of capillaries used, allowing one to run more samples per injection while reducing the time of a run.
The abi 3xl is a 16 capillary electrophoresis system that uses fluorescently labeled dyes for detection of dna. Jan 18, 2006 the 3 genetic analyzer is similar to the 3100avante as it uses a 4 capillary array i. Gene testing instrument capillary sequencing abi 3xl. Hitachi abi prism 3xl genetic analyzer 16 capillary unit with computerkey features 16 capillaries for the 3xl and 4 capillaries for the 3 system 24hour unattended operation easy instrument setup polymer filling via the automated polymer deliver system detection cell heater for improved thermal control automated sample injection from both 96. The applied biosystems 3 and 3xl genetic analyzers includes patented technology licensed from hitachi, ltd. Applied biosystems 310 genetic analyzer data collection software preferences module windows nt, 2000. Hitachi abi prism 3xl genetic analyzer 16 capillary unit with computer key features 16 capillaries for the 3xl and 4 capillaries for the 3 system 24hour unattended operation easy instrument setup polymer filling via the automated polymer deliver system detection cell heater for improved thermal control automated sample injection from both 96. Software for dna sequencingbase calling sequencing analysis v5. Browse our useful resources and support for your 3 or 3xl genetic analyzer. Abi 3xl genetic analyzer the applied biosystems 3xl genetic analyzer.
Find useful tips for working with your 33xl genetic analyzer from setting up. Please be aware that the pc computer cannot be included in the sale with this item. Applied biosystems 310 genetic analyzer support thermo. Battery backup ups for applied biosystems 33xl genetic.
Genetic analyzers include software for data collection and autoanalysis of sample files. Upgrade of applied biosystems genetic analyzers, 3500. Here is a good working applied biosystems hitachi 3xl genetic analyzer dna sequencer. There are many differences between the two genetic analyzers, the 3500xl genetic analyzer and 3xl genetic analyzer being compared in this validation study. Applied biosystems 3xl genetic analyzer is the latest generation of 16capillary electrophoresis instruments for the medium throughput laboratories. The analysis software uses the size standard to create a standard curve for each lane and then determines. Abi 3 genetic analyzersequencer certified scientific. Hitachi abi prism 3xl genetic analyzer 16 capillary unit with computer key features 16 capillaries for the 3xl and 4 capillaries for the 3 system 24hour unattended operation easy instrument setup polymer filling via the automated polymer deliver system detection cell heater for improved thermal control automated sample injection from both 96 and 384well.
Software the applied biosystems 3 and 3xl genetic analyzers include software for data collection and autoanalysis of sample files. The 3 genetic analyzer is similar to the 3100avante as it uses a 4 capillary array i. Abi 3xl genetic analyzer protocols for genotyping dna samples by marla hall adapted from kansas state university genotyping lab the abi 3xl is a 16 capillary electrophoresis system that uses fluorescently labeled dyes for detection of dna. Applied biosystems 3 or 3xl genetic analyzer power. Rfid system of 3500 series which performs automatic detection of reagents and instrument accessories used, forces the instrument operators to replace these reagents and accessories unnecessarily frequently, nonefficiently and makes the whole operation of 3500 series very expensive. Hitachiapplied biosystems genetic analyzer 3xl seller added the following information on 11282017 2. The system offers industryleading performance, plus sophisticated automation capabilities allowing researchers to save time, reduce costs and increase productivity. The applied biosystems 16capillary 3xl genetic analyzer is used for sanger sequencing and fragment analysis applications including microsatellite analysis, aflp, loh, snp validation and snp screening.
System components the 3100 genetic analyzer system includes the following components. Obtain access to useful resources and answers to commonly asked questions regarding the 310 genetic analyzer including instrument maintenance and usage information. Software loaded for specific instrument operation the ups is loaded with software settings that limit the voltage fluctuations to within the range specified. Applied biosystems hitachi abi prism 3 genetic analyzer computer software cables and connectors description with the applied biosystems 3 genetic analyzer, you get the sophisticated automation and superior performance of the 3 system, at acquisition and operating costs tailored to a growing research lab. Microsatellite analysis on the applied biosystems 3. On going validation of the 3500xl genetic analyzer using. Rather than risking inconsistency as a result of pouring slabgels, a simple capillary array is fit into the slots available.
This tradition of pioneering and innovation in the field of genetic analysis continues with the. Software abi 33xl genetic analyzer presets software for data collection and automatic analysis of sample files. As such, simply changing the computer name will lead to errors in the operation of the 3xl and its associated software. Samplefile analysis is performed with sequencing analysis software v5. It uses windows xp operating system, and sequencing analysis software v5. Collection software version user manual for the acceptance criteria recommended by applied biosystems. Identifiler analysis on the abi 3xl genetic analyzer. Fluorescently labeled fragments are detected using the applied biosystems 3xl genetic analyzer and then interpreted using the genemapper analysis software. Abi 3xl dna sequencer the abi 3xl dna sequencer is a 16capillary instrument that offers highquality data, efficient sample processing, and 24hour unattended operation. Find and purchase 3xl genetic analyzer products at invitrogen life science technologies. Applied biosystems 3 genetic analyzer thermo fisher. Applied biosystems 3 3xl genetic analyzers support.
Used applied biosystems 3xl genetic analyzer 2030217. Identifiler analysis on the abi 3xl genetic analyzer a. Used applied biosystems 3xl genetic analyzer 2020339. System is complete with computer and original disks. The multicapillary abi 3100 genetic analyzer became available in 2000 and enabled higher throughputs compared to the singlecapillary abi 310 instrument.
The 3xl genetic analyzer is the latest generation of 16capillary electrophoresis instruments for the medium throughput laboratories. User should be administrator, password should be left blank. The applied biosystems 3xl is a 16capillary electrophoresis system for genetic analysis. Browse our useful resources and support for your 3 or 3xl genetic analyzer including setup and maintenance as well as troubleshooting advice. The 3xl 16capillary instrument became available in 2003 table 6. Five different colored fluorescent dyes can be detected in one sample. We recommend that you contact an equipnet sales representative to set up your own inspection. Abiprism 3100 genetic analyzer definition the abiprism 3100 genetic analyzer is an automated capillary electrophoresis system that can separate, detect, and analyze up to 16 capillaries of fluorescently labeled dna fragments in one run. Improper boot sequence can lead to communication issues, aborted runs, and possible loss of data. Installation of the data collection software for the applied biosystems 33 xl genetic analyzer is a complex process that requires a field service engineer to install the software properly. Of course, one can perform a complete uninstall of all abi related software, change the computer name, and then reinstall the abirelated software. And 3xl genetic analyzer genemapper software and pop7 polymer users manual for limited label license or disclaimer information. Move the 3xl mtdna files from the lims fileshare l.
This system is designed to provide voltage regulation, surge suppression, and automatic emergency backup power specifically for the applied biosystems 3 or 3xl genetic analyzer. Upgrade of applied biosystems genetic analyzers, 3500 series. There are four main steps required to genotype samples. There are four main steps required to genotype samples using the abi 3xl, 1 pcr, 2 abi plate setup, 3 run on abi machine, and 4 data analysis using genetic software. Feb 10, 2016 abi 3xl sequencer available for sale at the lab world group. The applied biosystems 3 and 3 xl genetic analyzers include patented technology licensed from hitachi, ltd. The abi 3 and 3xl genetic analyzers are the newest in a range of analyzers produced by abi. Applied biosystems 3500 genetic analyzer from thermo. These machines use capillary electrophoresis, which is a significant advantage over slabgel based technologies.
Applied biosystems 33xl genetic analyzers site preparation guide pn 4352717 contains the space, environmental, and electrical requirements to support the 33xl genetic analyzer system. Applied biosystems 37303730xl dna analyzers and applied biosystems 33xl genetic analyzers ab navigator software administrator guide. Applied biosystems 37303730xl dna analyzers and 3 3xl genetic analyzers ab navigator software administrator guide. Applied biosystems 33xl genetic analyzers quick reference card pn 4362825 contains a flowchart on how to run the samples and instrument, a table of maintenance tasks, and a data collection software reference guide. Applied biosystems, genemapper, and liz are registered trademarks and ab design, applera, genescan, hidi, iscience, iscience design, pop, and. Abi 3xl sequencer available for sale at the lab world group.
Applied biosystems hitachi abi prism 3 genetic analyzer. Perform the spectral calibration as described in the abi 3 3xl genetic analyzer user manual with the following modifications. Applied biosystems 3 genetic analyzer applied biosystems 3xl genetic analyzer. Applied biosystems 3500 genetic analyzer from thermo fisher. Microsatellite analysis on the applied biosystems 3 series.
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